Cell Theory and Structure - Susquehanna University
The Microscope and Cell Theory The Microscope An understanding of cells and the ultrastructure of cells had to wait until objects so small could be visualized by magnification. Origin of the Microscope The first record of the invention of a microscope was by Hans and Zacharias Janssen (Netherlands) in
1590? Galileo Galilei improved the microscope in 1609 (in the same way that he improved the telescope) and by 16241625 published magnified drawings of insects. Suffered from chromatic and spherical aberrations Galileos first microscope from 1609
Illustrations of honey bees made with an improved Galileo microscope in 1630 Antonie Philips van Leeuwenhoek Made a powerful single-lens microscope with which he observed many types of unicells and tissues. He even drew bacteria.
A single-lens microscope 1632-1723, Netherlands His drawing of an ash stem section Robert Hookes Micrographia Hooke (1635-1703, UK) was intrigued by the reports sent by
van Leeuwenhoek and built his own microscope on the design of Galileo He confirmed many of the observations that van Leeuwenhoek reported Made many of his own original observations Published Micrographia in 1665 Much of the microscopic work of the rest of the 17th
and 18th centuries was descriptive [and there was much to describe]. Near the beginning of the 19th century, microscopes were made that reduced the problems of chromatic aberration Trunnion Model microscope made by Charles A. Spencer (1813-1881), US manufacturer
of microscopes Ernst Karl Abbe Partner of Carl Zeiss Optical physicist Invented the apochromatic lens and corrections to spherical aberration Sought to maximize resolution of the light microscope Demonstrated the limit of
resolution by visible light around 0.5mm 1840-1905, Germany The Cell Theory also, by the early part of the 19th century enough biological material had been observed to begin to make generalizations. Matthias Jakob Schleiden A botanist and microscopist In 1838 declared that the
plant organism is made of cells He accepted the importance of the nucleus, discovered by Robert Brown (1773-1858), and speculated on its role in cell division 1804-1881, Germany Theodor Schwann
He was a zoologist and a microscopist Physiologist and microanatomist Discovered the connection between yeast and fermentation Following Schleidens pronouncement, declared that animals, too, are made of cells as the fundamental building blocks
1810-1882, Germany Virchow and Remak Rudolph Carl Virchow (1821-1902, Germany) suggested that disease is a cellular problem He stated that all cells come from pre-existing cells, a theory stated earlier by Robert Remak
(1815-1865, Poland) Virchows cell theory Virchow (1821-1902) Remak (1815-1865) The Cell Theory by 1858 All living organisms are composed of one or more cells.
The cell is the most basic unit of structure, function, and organization in all organisms. All cells come from pre-existing, living cells. Histology and Paul Ehrlich Aniline dyes had been derived from coal tars in the early part of 19th Century Karl Weigert (1845-1904, Germany) used an aniline dye to stain bacteria Ehrlich experimented with
staining human tissues He used chemical treatments to serve as the first chemotherapy (used Methylene Blue for syphilis) 1854-1915, Germany The use of aniline dye to stain white blood cells
Development of Optical Staining Bright field Phase-Contrast Polarized light Differential Interference Contrast Darkfield
Fluorescence Imaging Electron Microscopy Two types: Transmission (TEM) and Scanning (SEM) Transmission Electron Microscopy Leo Szilard (1928) filed patent for electron microscope in concept The prototype built in 1931 by
Ruska and Knoll Resolution limit for light microscope ~2,000X; but the limit for TEM ~2 millionX Max Knoll (1897-1969, Germany, left) and Ernst Ruska(1906-1988, Germany, right) with first TEM Scanning Electron Microscopy Vladimir Kosmich Zworykin (1888-1982, Russia and
USA) invented SEM and television (both based on raster principle) With early television (kinetoscope) in 1929 Current View of the Cell Theory All known living things are made up of one or more cells. All living cells arise from pre-existing cells by division. The cell is the fundamental unit of structure and function in all living organisms.
The activity of an organism depends on the total activity of independent cells. Energy flow occurs within cells. Cells contain hereditary information (DNA) which is passed from cell to cell during cell division. All cells are basically the same in chemical composition in organisms of similar species.
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